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Ecosystem Fabrication (EcoFAB) controlled laboratory habitats

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Controlled laboratory habitats (EcoFABs) for mechanistic studies of plant-microbe interactions within specific environmental conditions. Two sizes of EcoFABs are described that are suited for the investigation of microbial interactions with various plant species, including Arabidopsis thaliana, Brachypodium distachyon, and Panicum virgatum. These flow-through devices allow for controlled manipulation and sampling of root microbiomes, root chemistry as well as imaging of root morphology and microbial localization.

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>Ecosystem Fabrication EcoFAB Jian Gao

Scientific article

Gao, J., Sasse, J., Lewald, K.M., Zhalnina, K., Cornmesser, L.T., Duncombe, T.A., Yoshikuni, Y., Vogel, J.P., Firestone, M.K., Northen, T.R., 2018. Ecosystem fabrication (EcoFAB) protocols for the construction of laboratory ecosystems designed to study plant-microbe interactions. J. Vis. Exp. 2018, 1–16. https://doi.org/10.3791/57170