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Sample holder with a flow cell connected to a constant-pressure perfusion and extraction system

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Contributor: Britta M C Kümpers (University of Nottingham) Number of printable pieces: 2 Recommended print material: Photocurable resin Licence: CC Attribution

Overview

3D-printed flow cell connected to a constant-pressure perfusion and extraction system to allow rapid changes between up to five growth and test media while maintaining the sample root orientated for imaging for prolonged periods on the stage of a confocal laser scanning microscope.

In action photo

Sample holder with a flow cell connected to a constant-pressure perfusion and extraction system Britta M C Kümpers

Scientific article

Kümpers, B. M. C., Han, J., Vaughan-Hirsch, J., Redman, N., Ware, A., Atkinson, J. A., Leftley, N., Janes, G., Castiglione, G., Tarr, P. T., Pyke, K., Voß, U., Wells, D. M., & Bishopp, A. (2021). Dual expression and anatomy lines allow simultaneous visualization of gene expression and anatomy. Plant Physiology. https://doi.org/10.1093/plphys/kiab503